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Given the hypothesis proposed regarding reduced glutathione biosynthesis around this timeframe within our study, conversion of excess GLY to other amino acids offers a plausible explanation

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The T94 2.61 I/E134 3.49 Q/M257 6.40 Y triple mutant, regenerated with 11- cis -retinal, and showed a main band at 378 nm with a minor contribution at 478 nm in the dark, compared to the characteristic visible absorbance band at 500 nm for the WT (Fig

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